diff --git a/backend/src/server/routes/index.ts b/backend/src/server/routes/index.ts
index 3952ea980..0cdc98791 100644
--- a/backend/src/server/routes/index.ts
+++ b/backend/src/server/routes/index.ts
@@ -281,7 +281,7 @@ export const registerRoutes = async (
     secretScanningDAL,
     secretScanningQueue
   });
-  const projectBotService = projectBotServiceFactory({ permissionService, projectBotDAL });
+  const projectBotService = projectBotServiceFactory({ permissionService, projectBotDAL, projectDAL });
 
   const projectMembershipService = projectMembershipServiceFactory({
     projectMembershipDAL,
diff --git a/backend/src/services/project-bot/project-bot-service.ts b/backend/src/services/project-bot/project-bot-service.ts
index 20419b610..f5298fdc2 100644
--- a/backend/src/services/project-bot/project-bot-service.ts
+++ b/backend/src/services/project-bot/project-bot-service.ts
@@ -113,6 +113,16 @@ export const projectBotServiceFactory = ({
     const { permission } = await permissionService.getProjectPermission(actor, actorId, bot.projectId, actorOrgId);
     ForbiddenError.from(permission).throwUnlessCan(ProjectPermissionActions.Edit, ProjectPermissionSub.Integrations);
 
+    const project = await projectBotDAL.findProjectByBotId(botId);
+
+    if (!project) {
+      throw new BadRequestError({ message: "Failed to find project by bot ID" });
+    }
+
+    if (project.version === "v2") {
+      throw new BadRequestError({ message: "Failed to set bot active, project has a default bot enabled" });
+    }
+
     if (isActive) {
       if (!botKey?.nonce || !botKey?.encryptedKey) {
         throw new BadRequestError({ message: "Failed to set bot active - missing bot key" });